Tom Packer
It was October 11, 2002 near Turner Valley, Alberta. There wasn’t much to go on. The lock on the gate leading into the field had been sheared with bolt cutters; there was a set of tire tracks in the snow, the head of a freshly killed cow moose and a gut pile. There were no witnesses and it seemed that nothing at the scene would assist the officer in identifying the person or persons responsible for the crime. Making the most of what little evidence he had, the officer took a meat sample from the moose head in the field. He knew that if his investigation led him to anyone with any part of that moose in their possession, the Alberta Fish and Wildlife Forensic Lab could use DNA typing to check for a link to the remains of the dead moose. The officer had few options so began contacting meat cutters in the Calgary and High River area to inquire if a cow moose had been delivered for processing. On Oct. 17, 2002 the officer learned that a cow moose had been delivered to a meat cutter for butchering. The officer inspected the moose and noted that the head appeared to have been cut off in the same fashion as the head he found in the field. There was a general moose tag attached to the carcass indicating the person responsible for killing the animal. A meat sample was collected from the moose for DNA analysis. Both meat samples that had been collected by the officer were sent to the Alberta Fish and Wildlife Forensic Lab. If the DNA typing profile (sometimes referred to as a DNA fingerprint) from the head matched the carcass at the meat cutters, he would know the samples came from the same moose. The officer would then be able to confirm that the individual named on the general moose tag was involved in the killing of the cow moose. If the DNA typing profiles did not match, then the officer would know that the head in the field and the moose that was hanging in the meat locker were from different animals, putting him back to square one with no witnesses and no leads. Dr. Rick Jobin, the scientist in charge of the DNA program at the lab has developed an effective test for DNA typing moose. Jobin had used this DNA test to analyze the genetics of hundreds of moose from three populations in Alberta as well as moose from the Yukon. This was exactly the kind of investigation that DNA labs are made for – no witnesses, no clues, and only a couple of samples of biological material. Unravelling the genetic codes in these meat samples was going to tell the story. Jobin carefully processed the samples. He extracted DNA, measured the quantity of DNA extracted, amplified small regions of the DNA in what is known as a polymerase chain reaction (PCR) process, then finally obtained the DNA typing profiles from each sample by measuring the size of the amplified regions of DNA. The results of the analysis were clear and unambiguous. The DNA typing profiles from the sample of the head at the kill site and the DNA typing profile from the sample of the moose at the meat cutter’s matched. Based on the analysis of the genetics of moose from three Alberta populations, Jobin was able to calculate the chances (random match probability) of two individual moose having this DNA typing profile was one in 103 billion. It was time for the officer to pay the person named on the moose tag a visit. Unfortunately, it would prove to be difficult because between the time the moose was killed and the time the DNA test results became available, the suspect had moved. The trail was cold. Refusing to give up, the officer contacted another enforcement agency to make an inquiry regarding the suspect. By chance the suspect was scheduled to appear in Calgary provincial court on unrelated matters. It was on that day that Calgary Fish and Wildlife officers charged the subject with Wildlife Act offences. Faced with DNA evidence from the lab, the man indicated that he would plead guilty to all charges and pay a fine. He admitted that the moose in question had been killed on the complainant’s property with a bow and that he did not possess a valid moose hunting licence for that area. His guilty pleas were accepted by the court and as a result he was fined. DNA typing is a powerful technology that Fish and Wildlife officers can use for the protection of wildlife populations. Investigators are now using DNA to develop leads in crimes with no witnesses and to turn dead end investigations into successful prosecutions.

Tom Packer is a forensic biologist 
with the Alberta Fish and Wildlife 
Division in Edmonton.